Chrysomya megacephala (Diptera: Calliphoridae) also known as oriental latrine fly is blow fly species widely distributed in many parts of the world. This fly has forensic importance as it infests on carrion soon after death and helps not only to estimate the post-mortem interval (PMI) but also to detect poisoning in a putrefying body. The present study was carried out on morphological identification of Chrysomya megacephala using key Lutz, 2018 and Cytochrome C Oxidase (COI). The special attention was focused on the tertiary morphological characteristics of flies collected from Nagpur region. The new COI subunit I (588 bp) gene sequence of Chrysomya megacephala have been added to GenBank (Accession No.- MN082633).