Diffusion-weighted magnetic resonance imaging (DW-MRI) based white matter tractography has become an indispensable tool in neuroscientific research and therapeutic neuroimaging. DSI Studio is a comprehensive diffusion MRI image reconstruction, fiber tracking, quantitative analysis and connectome mapping software package. DSI Studio offers quantitative anisotropy (QA) based tracking and supports a number of reconstruction models, including diffusion spectrum imaging (DSI), generalized q-sampling imaging (GQI) and diffusion tensor imaging (DTI), and is thus quite versatile. DSI Studio is a sophisticated software application designed for processing, analysing, and visualizing diffusion MRI (dMRI) data using Diffusion Spectrum Imaging (DSI) techniques. Fiber tractography is useful in clinical practice for surgical planning by neurosurgeons for avoiding important white matter tracts and lowering surgical risks. It helps wi.th abnormality detection, diagnosis support, planning of treatments, and insights into neurological diseases.