3D bioprinting has revolutionized manufacturing and design. Recent advances have enabled 3D printing of biocompatible materials, cells and supporting components into complex 3D functional living tissues. Inkjet printing, stereolithography (SLA), fused deposition modeling (FDM), selective laser sintering (SLS), and digital light processing (DLP) are prominent methods, each offering unique advantages. Materials used in 3D printing span plastics are PLA and ABS, metals, resins, ceramics, and other biocompatible substances. Scaffolds, crucial for supporting intricate structures during printing can be removable or water-soluble. In tissue engineering, the exploration of materials, nanoscale properties and the advancements in tissue decellularization methods offer opportunities for generating precise extracellular matrix (ECM) scaffolds with applications in regenerative medicine and bioprinting. Understanding natural compositions, striking a balance between removing cells and preserving structural integrity, and handling possible toxicity in cell culture on decellularized substrates are some of the factors to take into account. This review article explores the diverse materials used in 3D bioprinting and the role of scaffolds in the process.